State Key Laboratory of Quality Research in Chinese Medicine

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Efficacy evaluation

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  • Kind of traditional Chinese medicine for activating blood circulation (rhizoma zedoariae yanhusuo) breast cancer research

    New method for separation and purification temperature zedoary turmeric volatile oil of furan diene and gemma ketone: volatile oil as the effective parts of curcuma plants over the years has always been the hot spot of the research at home and abroad, its half terpene composition has anti-inflammatory and anti-cancer activity, more experiments using column chromatography to wen zedoary turmeric volatile oil chemical separation, furan diene 10 g, gemma ketone 1 g.

    In figure 1. WenYuJin separation furan diene and new methods of JiMa ketone Furan diene and tamoxifen combination study of effects of breast cancer: furan diene and commonly used breast cancer drug tamoxifen resistance joint application, the results showed that both have good synergy, prompt furan diene has great clinical application potential and prospects.

    Figure 2. Furan diene and tamoxifen together generate synergy effect against breast cancer (1) the MCF - 7 and MDA - MB - 231 breast cancer cell lines, furan diene can obviously enhance the antitumor effect of tamoxifen, the two together shows strong synergy. At the same time, furan diene and tamoxifen together for the cell cycle, cell apoptosis, have apparent effect. Figure 2. Furan diene and tamoxifen together generate synergy effect against breast cancer (1) the MCF - 7 and MDA - MB - 231 breast cancer cell lines, furan diene can obviously enhance the antitumor effect of tamoxifen, the two together shows strong synergy. At the same time, furan diene and tamoxifen together for the cell cycle, cell apoptosis, have apparent effect.

    Figure 3. Furan diene and tamoxifen together generate synergy effect of breast cancer resistance (2)

  • Righting thrift kind of traditional Chinese medicine (ganoderma lucidum) breast cancer research

    By modifying the method of preparation of a new ganoderma extract, after the test, the extraction of effective ingredients in ganoderma lucidum and improved. The breast cancer cell lines in vitro of extracts from the antitumor effect and possible mechanism has carried on the preliminary discussion. Studies have found that the extract could significantly inhibit the MCF - 7 cell proliferation, induce cells to produce the G1 stage of cell cycle arrest, DNA damage and produce.

    Figure 4. The extraction of ganoderma extract program

    Figure 5. Ganoderma lucidum extract induced MCF - 7 cell DNA damage and the G1 stage of cell cycle arrest

  • Based on the neovascularization of zebrafish screening study

    Screening using zebrafish model found some Chinese medicine extract and monomer compounds have good promote/antiangiogenic activity or nerve activity of protection. Among them, astragalus, angelica extract and monomer composition of TCM for activating blood circulation Calycosin separately (Calycosin) and astragalus glycosides (Astragaloside IV) has a good promoting angiogenesis activity; Found that traditional Chinese medicine (TCM) dried tangerine or orange peel extract and monomer composition of sichuan and dried tangerine or orange peel (Nobiletin), traditional Chinese medicine monomer compound indigo jade red (Indirubin) and Resveratrol (nods) and its derivatives (TMS) has a strong antiangiogenic activity. Neural activity screening found that Chinese medicine Fructus alpiniae oxyphyllae protection (Fructus Alpinia oxyphylla) extract, fine grass valley (Eriocaulon buergerianum) extract, quercetin (quercetin), monomer compounds Shi Sha alkaline division A derivatives [Bis (12) - hupyridone/huperzine A], ligustrazine derivatives (TBN, TN2), vascular endothelial growth factor receptor inhibitor 2 (VEGFR2) SU4312 and SU5416 in Parkinson's disease in zebrafish model and cell model with good neuroprotective activity. Using mass spectrometry detection of epimedium success element and a phase and two phase in the metabolic production of calycosin separately, combination of proteomics and transcriptome study method proves that zebrafish metabolic enzymes metabolic enzymes are highly conservative and mammals. Research from several aspects elaborated the epimedium flavin and its derivatives in the metabolic pathway of zebrafish whole model, distribution mechanism, zebrafish drug metabolism study provides a new train of thought. The zebrafish drug screening platform was chosen as the Macao science center organization "science around you vote your favorite local scientific research" one of the most popular research projects, was selected as one of the first exhibition project of establishment of the Macao science museum; Project implementation process of the communist party of China the training of specialists in the field of the related research field 16 people; 22 article published scientific papers, of which 2 paper was selected for the cover of the international journal of important papers; Scientific research conference papers won awards and Macao international and domestic several; Screening for angiogenic activity in the United States and angelica extract of Chinese medicine research institute (Sunny BioDiscovery) cooperation, conducted clinical trials, the results showed that angelica extract can effectively treat chronic diabetic foot ulcers. Traditional Chinese medicine of the activity of angiogenesis research project has been awarded to Macao young researcher award. Distinctions of IIM 's Young Researcher' s Award 2010 in Macau (On the field of Applied Science). The zebrafish angiogenic drug screening platform was selected as one of the first exhibition project of establishment of the Macao science museum. Cultivating professional talents of the angiogenic drug research areas, a total of three PhD, master's 13, including postgraduate students have graduated from nine; Were applied for the 2 invention patents of the People's Republic of China, the 17th international papers published articles, 5 papers domestic and macau, another research papers in the writing and contribute more. Related research summary and posters to attend academic conference for many times at home and abroad, including calycosin separately promote angiogenesis research to obtain the 13th annual meeting of China association for science and technology of biological medicine, Dr. BBS. Innovation talent award - the first prize, astragalus extract of research "the 4th Hong Kong and Macao graduate institute of traditional Chinese medicine" award for the best paper posters; Calycosin separately cardiovascular research study won "the eighth Chinese medicine globalization league BBS" organized the conference to provide full funding and access to "the 7th international graduate institute of traditional Chinese medicine" award for the best in the poster, calycosin separately metabolic research and ADTM pharmacological research "the 10th league BBS" TCM globalization which organized the conference to provide full funding, resveratrol and its derivatives research for "first world natural and traditional Chinese medicine pharmacology seminar" award for the best paper posters; Fine grass valley research "the fifth graduate institute of traditional Chinese medicine of Hong Kong and Macao" award for the best in the poster, the Macao daily newspaper reported it; The study of fructus alpiniae oxyphyllae won a Macao first doctoral student paper awards of BBS.

  • Based on the patch clamp screening study of traditional Chinese medicine

    The main purpose of this project is to build a whole cell patch clamp technique. Progress of the project is good, we are able to successfully operating H9C2 cardiac muscle cells of the whole cell patch clamp experiments to evaluate drug chemical voltage-gated potassium channels of various properties. The progress of the project, we face the difficulties of all sorts of problems with the patch clamp technique. The original advice of nerve types of cell lines is a kind of unstable cell line of technical difficulties. We filter the right cell experiment success. In this project, we have seen in the single compounds and a few types of Chinese herbal medicine, including radix astragali, radix scutellariae and total extract of salvia miltiorrhiza. We have by cell lines H9C2 study of the relationship with the potassium ion channel, whole-cell patch clamp is compound of protection. So far, we don't have any paper with the project, but we expect to see in the next six months, we will be able to release the file of the extract and potassium ion channel, the evaluation of single compound huangqi. The project to support a full-time graduate student and a part-time graduate student. It will also support another full-time graduate since November 2011. Students have the opportunity to learn from domain experts patch clamp technique and skills. The project's main purpose is to assess two structure of similar compounds, from the famous traditional Chinese medicine astragalus radix polygoni multiflori alleviate menopausal symptoms. The progress of the project is very good, and calycosin separately and the handle, the relationship in vascular endothelial cells and vascular smooth muscle are revealed in detail. We also look forward to in the blood vessels of TCM and different receptors in the system of the relationship between the vascular tissue is very important. In this project, we have seen in a single compound, calycosin separately and mans, the main handle. So far, we don't have any extensions with the project of the papers published, but we currently expect, in the next six months, we will be able to pharmacology of SCI papers published on blood vessels. This project has a master graduate student support. This project allows no pharmacology background of students to master and understand the theory behind the problem, and put into practice and research.

  • Gourd dildos trap study of element compounds determined by MTT

    In screening neural protection cell experiments, we found that the gourd element compounds can "protect" neurotoxin 6 - OHDA induced PC12 cell injury. And optical microscope found that cells form have changed, your cells has been damaged. B by cell count, the study found the gourd can significantly reduce the number of PC12 cells. JC - 1 fluorescent staining showed that CuB on PC12 cells had no effect on mitochondrial membrane potential, Hoechst 33342 dyeing is found no apoptotic body formation. In addition, the CuB on apoptosis related proteins Bax, Bcl - 2, Caspase - 7, 8, 9, and the expression of PARP has no obvious influence, for PC12 cells had no significant effect of the cell cycle, at the same time, the block element B of intracellular GSH, ATP content had no obvious effect. In PC12 cells, CuB processing can produce false-positive trap determined by MTT. CuB obvious cytotoxicity on PC12 cells and its mechanism has nothing to do with the induction of cell cycle arrest and apoptosis.

    Figure 6. Gourd B inhibit PC12 cell proliferation

    Figure 7. Gourd element B inhibit PC12 cell proliferation but does not affect cell mitochondrial membrane potential

    Figure 8. The study results national academic conference of the 11th Chinese pharmacology, won the award for outstanding posters.