Shaoping LI  李紹平
Professor
Deputy Director of ICMS & State Key Laboratory of Quality Research in Chinese Medicine (University of Macau)

Academic Qualifications | Teaching | Research | Selected Publications | Qualifications | Contact Details

Academic Qualifications

  • MSc, PhD, China Pharmaceutical University

Teaching

M.Sc. Courses

  1. Quality control (MCMC100)
  2. Development of New Drugs and Health Products (MCMC102)

Research

Research Interests

  • Extraction, isolation and determination of active ingredients, especially polysaccharides and volatile compounds, in Chinese medicines.
  • Quality control methods, including HPLC, LC-MS, CE, CEC and GC-MS, for Chinese medicines.
  • Active ingredients and quality control of medicinal mushroom, such as Cordyceps spp., Ganoderma spp..

Research Group

  • Postdoctoral Fellows

Qiao Chun-feng 喬春峰

  • Ph.D Students

Feng Kun 馮昆, Li Deqiang 李德強, Meng Lanzhen 孟蘭貞, Li Jing 李靜, Lv Guangping 呂廣萍, Chen Yiwen 陳藝文, Wang Lanying 王蘭英，Hu Dejun 胡德俊，Cheong Kit Leong 張傑良，Chen Xianqiang 陳顯強，Xie Jing 謝靜，Wu Dingtao 吳定濤

  • Master Students

Liu Xiaomei 劉曉妹，Liu Ge 劉戈，Liu Jiaming 劉嘉明，Lei Haipeng 雷海鵬，Jv Yaojun 巨瑤君，Cao Kaiyue 曹凱悅，Zong Wanrong 宗皖容，Yang Zhongmei 楊中梅 , Zhang Tianfang 張天方

Selected Publications (*Corresponding Author)

Books & Book Chapters

  1. Lin, B.Q., Li, S.P.* (2011) Cordyceps. In: Iris Benzie & Dr. Sissi Wachtel-Galor (eds.) Herbal Medicine: Biomolecular and Clinical Aspects. Taylor and Francis Group.
  2. Li, P., Li, S.P.* (2010). Pressurized Liquid Extraction in Phytochemical Analysis. In: Tzanavaras, P.D. & Zacharis, C.K. (eds.), REVIEWS IN PHARMACEUTICAL AND BIOMEDICAL ANALYSIS. Bentham Science Publishers, Netherlands, pp 130-148.
  3. Li, S.P., Wang, Y.T. (2009) Chromatographic Analysis for Commonly Used Chinese Materia Medica. University of Macau, Macao.
  4. Chen, X.J., Yang, F.Q. Li, S.P.* (2009) Application of Chemometrics in Capillary Electrophoresis Analysis of Herbal Medicines. In: Grady Hanrahan & Frank A. Gomez (eds.) Chemometric Methods in Capillary Electrophoresis. John Wiley & Sons, Inc., pp 227-242.
  5. Li, S.P., Wang, Y.T. (2008) Pharmacological Activity Based Quality Control of Chinese Herbs. Nova Science Publishers, Inc. New York.
  6. Yang, F.Q., Li, S.P.* (2008) Quality control of essential oil in Chinese medicine. In: Felix M. Ching. (eds.) Chinese Herbal Drug Research Trends. Nova Science Publishers, Inc. New York. pp 67–98.
  7. Guan, J., Li, S.P.* (2008) Quality control of polysaccharides from medicinal plants and fungi. In: Felix M. Ching. (eds.) Chinese Herbal Drug Research Trends. Nova Science Publishers, Inc. New York. pp 31–65.
  8. Zhao, J., Hong, W., Li, S.P. (2008) How to control heavy metals in Chinese medicines rationally?. In: Felix M. Ching. (eds.) Chinese Herbal Drug Research Trends. Nova Science Publishers, Inc. New York. pp 15–19.

Selected Journal Articles 

Study on Quality Control of Chinese Medicines

  1. Li, S.P., Zhao, J., Yang, B. (2011) Strategies for quality control of Chinese medicines. J. Pharm. Biomed. Anal. 55: 802-809.
  2. Chen, X. J., Zhao J., Wang Y. T., Huang L. Q., Li, S. P.* (2012) CE and CEC analysis of phytochemicals in herbal medicines. Electrophoresis 33: 168–179. (Invited review)
  3. Yang, F. Q., Zhao, J., Li, S. P.* (2010) CEC of Phytochemical Bioactive Compounds. Electrophoresis 31: 260-277. (Invited review)
  4. Chen, X. J., Yang, F. Q., Wang, Y. T., Li, S. P.* (2010) CE and CEC of nucleosides and nucleotides in food materials. Electrophoresis 31: 2092-2105. (Invited review)
  5. Li, P., Li, S.P.*, Wang Y.T. (2006) Optimization of capillary zone electrophoresis for analysis of phytochemical bioactive compounds. Electrophoresis 27: 4808–4819 (Invited review).
  6. Zhao, J., Lv, G. P., Chen, Y. W., Li, S. P.* (2011) Advanced development in analysis of phytochemicals from medicine and food dual purposes plants used in China. J. Chromatogr. A 1218: 7453-7475. (Invited review)
  7. Wan, J. B., Zhang, Q. W., Hong, S. J., Li, P., Li, S. P.*, Wang, Y. T.* (2012) Chemical investigation of saponins in different parts of Panax notoginseng by pressurized liquid extraction and liquid chromatography-electrospray ionization-tandem mass spectrometry. Molecules 17: 5836-5853.
  8. Chen, X. J., Dinh, N. P., Zhao, J., Wang, Y. T., Li, S. P.*, Svec, F. (2012) Effect of ion adsorption on CEC separation of small molecules using hypercrosslinked porous polymer monolithic capillary columns. J. Sep. Sci. 35: 1502-1505.
  9. Calway, T., Du, G. J., Wang, C. Z., Huang, W. H., Zhao, J., Li, S. P.*, Yuan, C. S. (2012) Chemical and pharmacological studies of Oplopanax horridus, a North American botanical. J. Nat. Med. 66: 249-256.
  10. Zhou, B., Tan, M., Lu, J.F., Zhao, J., Xie, A.F. Li, S.P.* (2012) Simultaneous determination of five active compounds in Chimonanthus nitens by double-development HPTLC and scanning densitometry. Chem. Cent. J. 6: 46.
  11. Xu, J., Guan, J., Chen, X.J., Zhao, J., Li, S.P.* (2011) Comparison of polysaccharides from different Dendrobium using saccharide mapping. J. Pharm. Biomed. Anal. 55: 977-983.
  12. Yang, J., Choi, L.L., Li, D.Q., Yang, F.Q., Zeng, L.J., Zhao, J., Li, S.P.* (2011) Simultaneous analysis of hydrophilic and lipophilic compounds in Salvia miltiorrhiza by double-development HPTLC and scanning densitometry. J. Planar Chromatogr.—Modern TLC 24: 257-263.
  13. Huang, W.H., Zhang, Q.W., Meng, L.Z., Yuan, C.S., Wang, C.Z., Li, S.P.* (2011) Oplopanphesides A-C, three new phenolic glycosides from the root barks of Oplopanax horridus. Chem. Pharm. Bull. 59: 676-679.
  14. Guan, J., Li, S. P.* (2010) Discrimination of polysaccharides from traditional Chinese medicines using saccharide mapping — enzymatic digestion followed by chromatographic analysis. J. Pharm. Biomed. Anal. 51: 590-598.
  15. Yang, C., Guan, J., Zhang, J. S., Li, S. P.* (2010) Discrimination of crude polysaccharides from six traditional Chinese medicines using high-performance thin-layer chromatography. J. Planar Chromatogr. – Modern TLC 23: 46-49.
  16. Huang, W. H., Yang, J., Zhao, J., Wang, C. Z., Yuan, C. S., Li, S. P.* (2010) Quantitative analysis of six polyynes and one polyene in Oplopanax horridus and Oplopanax elatus by pressurized liquid extraction and on-line SPE-HPLC. J. Pharm. Biomed. Anal. 53: 906-910.
  17. Lv, G. P., Huang, W. H., Yang, F. Q., Li, J., Li, S. P.* (2010) Pressurized liquid extraction and GC-MS analysis for simultaneous determination of seven components in Cinnamomum cassia and the effect of sample preparation. J. Sep. Sci. 33: 2341–2348.
  18. Xu, J., Zhao, W. M., Qian, Z. M., Guan, J., Li, S. P.* (2010) Fast determination of five components of coumarin, alkaloids and bibenzyls in Dendrobium species using pressurized liquid extraction and ultra-performance liquid chromatography. J. Sep. Sci. 33: 1580–1586.
  19. Zhong, G.X., Li, P., Zeng, L.J., Guan, J., Li, D.Q., Li, S.P.* (2009) Chemical characteristics of Salvia miltiorrhiza (Danshen) collected from different locations in China. J. Agric. Food Chem. 57: 6879–6887.
  20. Qian, Z.M., Lu, J., Gao, Q.P., Li, S.P.* (2009) Rapid method for simultaneous determination of flavonoid, saponins and polyacetylenes in Folium Ginseng and Radix Ginseng by pressurized liquid extraction and high-performance liquid chromatography coupled with diode array detection and mass spectrometry. J. Chromatogr. A 1216: 3825–3830.
  21. Jiang, Y., Li, S.P., Wang, Y.T., Chen, X.J., Tu, P.F. (2009) Differentiation of Herba Cistanches by fingerprint with high-performance liquid chromatography–diode array detection–mass spectrometry. J. Chromatogr. A 1216: 2156–2162.
  22. Xie, J.J., Lu, J., Qian, Z.M., Yu, Y., Duan, J.A., Li, S.P.* (2009) Optimization and comparison of five methods for extraction of coniferyl ferulate from Angelica sinensis. Molecules 14: 555–565.
  23. Chen, X.J., Tu, P.F., Jiang, Y., Wang, Y.T., Li, S.P.* (2009) Effect of stability of internal standard on quantification of 15 flavonoids in Epimedium using CZE. J. Sep. Sci. 32: 275–281.
  24. Qian, Z.M., Wan, J.B., Zhang, Q.W., Li, S.P.* (2008) Simultaneous determination of nucleobases, nucleosides and saponins in Panax notoginseng using multiple columns high performance liquid chromatography. J. Pharm. Biomed. Anal. 48: 1361–1367.
  25. Zhang, J.S., Guan, J., Yang, F.Q., Liu, H.G., Cheng, X.J., Li, S.P.* (2008) Qualitative and quantitative analysis of four species of Curcuma rhizomes using twice development thin layer chromatography. J. Pharm. Biomed. Anal. 48: 1024–1028.
  26. Gao, X.H., Zhang, Q.W., Li, S.P.* (2008) Preparative separation of three compounds from essential oil of Curcuma longa using high performance centrifugal partition chromatography. Asian Chem. Lett. 12: 81–86.
  27. Chen, X.J., Ji, H., Wang, Y.T., Li, S.P.* (2008) Simultaneous determination of seven flavonoids in Epimedium using pressurized liquid extraction and capillary electrochromatography. J. Sep. Sci. 31: 881–887.
  28. Li, P., Xu, G., Li, S.P., Wang, Y.T., Fan, T.P., Zhao, Q.S., Zhang, Q.W. (2008) Optimizing ultraperformance liquid chromatographic analysis of 10 diterpenoid compounds in Salvia miltiorrhiza using central composite design. J. Agric. Food Chem. 56: 1164–1171.
  29. Chen, X.J., Ji, H., Zhang, Q.W., Tu, P.F., Wang, Y.T., Guo, B.L., Li, S.P.* (2008) A rapid method for simultaneous determination of 15 flavonoids in Epimedium using pressurized liquid extraction and ultra-performance liquid chromatography. J. Pharm. Biomed. Anal. 46: 226–235.

Study on medicinal mushroom

  1. Lv, G. P., Zhao, J., Duan, J. A., Tang, Y. P., Li, S. P.* (2012) Comparison of sterols and fatty acids in two species of Ganoderma. Chem. Cent. J. 6: 10.
  2. Xie, J., Zhao, J., Hu, D. J., Duan, J. A., Tang, Y. P., Li, S.P.* (2012) Comparison of polysaccharides from two species of Ganoderma. Molecules 17: 740-752.
  3. Yang, J., Chen, Y. W., Leong, N. I., Zhao, J., Duan, J. A., Tang, Y. P., Li, S.P.* (2012) Quality evaluation of different products derived from Ganoderma. J. Med. Plant Res. 6:1969-1974.
  4. Guan, J., Zhao, J., Feng, K., Hu, D.J., Li, S.P.* (2011) Comparison and characterization of polysaccharides from natural and cultured Cordyceps using saccharide mapping. Anal. Bioanal. Chem. 399: 3465-3474.
  5. Yang, F. Q., Li, D. Q., Feng, K., Hu, D. J., Li, S. P.* (2010) Determination of nucleotides, nucleosides and their transformation products in Cordyceps by ion-pairing reversed-phase liquid chromatography-mass spectrometry. J. Chromatogr. A 1217: 5501–5510.
  6. Guan, J., Yang, F. Q., Li, S. P.* (2010) Carbohydrates in Natural and Cultured Cordyceps Evaluated by Pressurized Liquid Extraction and Gas Chromatography Coupled with Mass Spectrometry. Molecules 15: 4221-4227.
  7. Feng, K., Wang, S., Hu, D.J., Yang, F.Q., Wang, H.X., Li, S.P.* (2009) Random amplified polymorphic DNA (RAPD) analysis and the nucleosides assessment of fungal strains isolated from natural Cordyceps sinensis. J. Pharm. Biomed. Anal. 50: 522–526.
  8. Wang, S., Yang, F.Q., Feng, K., Li, D.Q., Zhao, J., Li, S.P.* (2009) Simultaneous determination of nucleosides, myriocin, and carbohydrates in Cordyceps by high-performance liquid chromatography coupled with diode array detection and evaporative light scattering detection. J. Sep. Sci. 32: 4069-4076.
  9. Yang, F.Q., Ge, L., Yong, J.W.H., Tan, S.N., Li, S.P.* (2009) Determination of nucleosides and nucleobases in different species of Cordyceps by capillary electrophoresis–mass spectrometry. J. Pharm. Biomed. Anal. 50: 307–314.
  10. Yang, F.Q., Feng, K., Zhao, J., Li, S.P.* (2009) Analysis of sterols and fatty acids in natural and cultured Cordyceps by one-step derivatization followed with gas chromatography–mass spectrometry. J. Pharm. Biomed. Anal. 49: 1172–1178.
  11. Yang, F.Q., Li, S.P.* (2008) Effects of sample preparation methods on the quantification of nucleosides in natural and cultured Cordyceps. J. Pharm. Biomed. Anal. 48: 231–235.
  12. Yang, F.Q., Feng, K., Li, S.P.* (2008) Quality evaluation of cultured Cordyceps sinensis with multiple pharmacological activities. Asian Chem. Lett. 12: 23–32.
  13. Fan, H., Yang, F.Q., Li, S.P.* (2007) Determination of purine and pyrimidine bases in natural and cultured Cordyceps using optimum acid hydrolysis followed by high performance liquid chromatography. J. Pharm. Biomed. Anal. 45: 141–144.
  14. Yang, F.Q., Guan, J., Li, S.P.* (2007) Fast simultaneous determination of fourteen nucleosides and nucleobases in cultured Cordyceps using ultra-performance liquid chromatography. Talanta 73: 269–273.
  15. Yu, L., Zhao, J., Zhu, Q., Li, S.P.* (2007) Macrophage biospecific extraction and high performance liquid chromatography for hypothesis of immunological active components in Cordyceps sinensis.J. Pharm. Biomed. Anal. 44: 439–443.
  16. Yang, F.Q., Li, S.P.*, Li, P., Wang, Y.T. (2007) Optimization of CEC for simultaneous determination of eleven nucleosides and nucleobases in Cordyceps using central composite design. Electrophoresis 28: 1681–1688.
  17. Li, S.P.*, Yang, F.Q., Tsim, K.W.K. (2006) Quality control of Cordyceps sinensis, a valued traditional Chinese medicine. J. Pharm. Biomed. Anal. 41: 1571–1584 (Invited review).
  18. Li, S.P.*, Zhang, G.H., Zeng Q., Huang, Z.G., Wang, Y.T., Dong, T.T.X., Tsim, K.W.K. (2006) Hypoglycemic activity of polysaccharide, with antioxidation, isolated from cultured Cordyceps mycelia. Phytomedicine 13: 428–433.
  19. Fan, H., Li, S.P.*, Xiang, J.J., Lai, C.M., Yang, F.Q., Gao, J.L., Wang, Y.T. (2006) Qualitative and quantitative determination of nucleosides, bases and their analogues in natural and cultured Cordyceps by pressurized liquid extraction and high performance liquid chromatography–electrospray ionization tandem mass spectrometry (HPLC–ESI–MS/MS). Anal. Chim. Acta. 567: 218–228.
  20. Yu, L., Zhao, J., Li, S. P.*, Fan, H., Hong, M., Wang, Y.T., Zhu, Q. (2006) Quality evaluation of Cordyceps through a simultaneous determination of eleven nucleosides and bases by RP-HPLC. J. Sep. Sci. 29: 953–958.
  21. Cheung, J.K.H., Li, S.P., Tsim, K.W.K. (2005) Authentication and quality control of Cordyceps sinensis, a traditional Chinese medicine known as winter-worm summer-grass. OPEM 5: 262–271.
  22. Li, S.P.*, Song, Z.H., Dong, T.T.X., Ji, Z.N., Lo, C.K., Zhu, S.Q., Tsim, K.W.K. (2004) Distinction of water-soluble constituents between natural and cultured Cordyceps by capillary electrophoresis. Phytomedicine 11: 684–690.
  23. Gong, Y.X., Li, S.P.*, Li, P., Liu, J.J., Wang, Y.T. (2004) Simultaneous determination of six main nucleosides and bases in natural and cultured Cordyceps by capillary electrophoresis. J. Chromatogr. A 1055: 215–221.
  24. Li, S.P.*, Li, P., Lai, C.M., Gong, Y.X., Kan, K.K.W., Dong, T.T.X., Tsim, K.W.K., Wang, Y.T. (2004) Simultaneous determination of ergosterol, nucleosides and their bases from natural and cultured Cordyceps by pressurised liquid extraction and high-performance liquid chromatography. J. Chromatogr. A 1036: 239–243.
  25. Li, S.P., Zhao, K.J., Ji, Z.N., Song, Z.H., Dong, T.T.X., Lo, C.K., Cheung, J.K.H., Zhu, S.Q., Tsim, K.W.K. (2003) A polysaccharide isolated from Cordyceps sinensis, a traditional Chinese medicine, protects PC12 cells against hydrogen peroxide-induced injury. Life Sci. 73: 2503–2513.
  26. Li, S.P., Su, Z.R., Dong, T.T.X., Tsim, K.W.K. (2002) The fruiting body and its caterpillar host of Cordyceps sinensis show close resemblance in main constituents andanti-oxidation activity. Phytomedicine 9: 319–324.
  27. Li, S.P., Li, P., Dong, T.T.X., Tsim, K.W.K. (2001) Anti-oxidation activity of different types of natural Cordyceps sinensis and cultured Cordyceps mycelia. Phytomedicine 8: 207–212.
  28. Li, S.P.,Li, P., Dong, T.T.X., Tsim, K.W.K. (2001) Determination of nucleosides in natural Cordyceps sinensis and cultured Cordyceps mycelia by capillary electrophoresis. Electrophoresis 22: 144–150.

Bioassay & Bioactive Compounds

  1. Jin H. R., Zhao J., Zhang Z., Liao Y., Wang C. Z., Huang W. H., Li S. P., He T. C., Yuan C. S., Du W. (2012) The antitumor natural compound falcarindiol promotes cancer cell death by inducing endoplasmic reticulum stress. Cell Death Dis. 3: e376.
  2. Ling, J., Wei, B., Lv, G.. P., Ji, H., Li, S. P.* (2012) Anti-hyperlipidaemic and antioxidant effects of turmeric oil in hyperlipidaemic rats. Food Chem. 130: 229-235.
  3. Li, D. Q., Qian, Z. M., Li, S. P.* (2010) Inhibition of Three Selected Beverage Extracts on a-glucosidase and Rapid Identification of Their Active Compounds Using HPLC-DAD-MS/MS and Biochemical Detection. J. Agric. Food Chem. 58: 6608–6613.
  4. Liu, Y.W., Gao, J.L., Guan, J., Qian, Z.M., Feng, K., Li, S.P.* (2009) Evaluation of antiproliferative activities and action mechanisms of extracts from two species of Ganoderma on tumor cell lines. J. Agric. Food Chem. 57: 3087–3093.
  5. Qian, Z.M., Guan, J., Yang, F.Q., Li, S.P.* (2008) Identification and quantification of free radical scavengers in Pu-erh tea by HPLC-DAD-MS coupled online with 2,2′-azinobis(3-ethylbenzthiazolinesulfonic acid) diammonium salt assay. J. Agric. Food Chem. 56: 11187–11191.
  6. Xiao, Y., Yang, F.Q., Li, S.P., Hu, G., Lee, S.M.Y., Wang, Y.T. (2008) Essential oil of Curcuma wenyujin induces apoptosis in human hepatoma cells. World J. Gastroenterol. 14: 4309–4318.
  7. Wang, J., Guang, Z.G., Cao, H., Wang, Y.T., Hui, P., Hoo, C., Li, S.P.* (2008) Screening of anti-platelet aggregation agents from Panax notoginseng using human platelet extraction and HPLC–DAD–ESI-MS/MS. J. Sep. Sci. 31: 1173–1180.
  8. Xiao, Y., Yang, F.Q., Li, S.P., Gao, J.L., Hu, G., Lao, S.C., Conceição, E.L., Fung, K.P., Wang, Y.T., Lee, S.M. (2007) Furanodiene induces G(2)/M cell cycle arrest and apoptosis through MAPK signaling and mitochondria-caspase pathway in human hepatocellular carcinoma cells. Cancer Biol Ther. 6: 1044–1050.
  9. Li, S.Y., Yu, Y., Li, S.P.* (2007) Identification of antioxidants in essential oil of Radix Angelicae sinensis using HPLC coupled with DAD-MS and ABTS-based assay. J. Agric. Food Chem. 55: 3358–3362.
  10. Dong, Z.B., Li, S.P.*, Hong, M., Zhu, Q. (2005) Hypothesis of potential active components in Angelica sinensis by using biomembrane extraction and high performance liquid chromatography. J. Pharm. Biomed. Aanl. 38: 664–669.

Qualifications

  • Editorial Advisory Board Member, Journal of Pharmaceutical and Biomedical Analysis
  • Editorial Board Member, Journal of Separation Science
  • Editorial Board Member, American Journal of Chinese Medicine
  • Editorial Board Member, International Journal of Analytical Chemistry
  • Associate Editor, Journal of Medicinal Plants Research
  • Editorial Board Member, International Journal of Essential Oil Therapeutics
  • Editorial Advisory Board Member, The Open Bioactive Compounds Journal
  • Editorial Board Member, International Journal of Biomedical and Pharmaceutical Sciences
  • Editorial Board Member, Medicinal and Aromatic Plant Science and Biotechnology
  • Editorial Board Member, Functional Plant Science and Biotechnology
  • Editorial Board Member, The Asian and Australasian Journal of Plant Science and Biotechnology
  • Editorial Board Member, Africa Journal of Agriculture Research
  • Editorial Board Member, Acta Pharmaceutica Sinica B
  • Editorial Board Member, Journal of Pharmaceutical Analysis
  • Editorial Board Member, Chinese Pharmaceutical Journal
  • Editorial Board Member, China Journal of Chinese Materia Medica
  • Editorial Board Member, Chinese Journal of Pharmaceutical Analysis
  • Associate Chairman, The Committee of Natural Medicinal Resources, China Society of Natural Resources
  • Committee Member, The Committee of Pharmaceutical Analysis, Chinese Pharmaceutical Association
  • Committee Member, The Committee of Chinese and Natural Medicines, Chinese Pharmaceutical Association
  • Standing Council Member, Sub-society of Industrial Biochemistry and Molecular Biology, The Chinese Society of Biochemistry and Biology
  • Senior member, Chinese Pharmaceutical Association
  • Member, Good Practice in Traditional Chinese Medicine Research in the Post-genomic Era (GP-TCM)



Contact Details

Institute of Chinese Medical Sciences
University of Macau
Macao, China

Room: 202A, 2F, Block 3
Telephone: (853) 8397-4692
Fax: (853) 28841358
E-mail:spli@umac.mo